Variants、eQTL、MPRA

本文內容主要來自 Barak Cohen 教授給的數堂課的筆記,以 Systems Biology 的角度來看 coding/noncoding variant modeling 和相關實驗 MPRA。

Ensembl Genomic Reference in Bioconductor

Using fundamental R/Biocondcutor packages (e.g. AnnotationHub, ensembldb and biomaRt) to query Ensembl genomic references or annotations.

Plot Sequencing Depth with Gviz

TL;DR Plot exome sequencing depth and coverage with genome annotation using Gviz in R. Then apply detail control on Gviz annotation track displaying.

This is an extending post from Genomic Data Processing in Bioconductor, though I haven’t finished reading all the reference in that post. The background knowledge …

Overview of Genomic Data Processing in Bioconductor

Notes of fundamental tools and learning resources for handling genomic data in R with Bioconductor.

FASTA/Q sequence processing toolkit -- seqtk

This post demonstrates the FASTQ to FASTA conversion and sequence quality check using seqtk.